STORAGE OF FUNGI USING STERILE DISTILLED WATER OR LYOPHILIZATION - COMPARISON AFTER 12 YEARS

The methods used in our laboratory for maintaining culture collections of fungi, i.e. storage in sterile distilled water or lyophilization, were evaluated. Seventy-eight isolates belonging to seven genera were tested. After 12 years of preservation, survival rates associated with distilled water storage and lyophilization were 89.7% and 87.2% respe ctively. The viability of the dermatophytes after 8 years was confirme d using urease test media, the hair penetration test and dermatophyte test medium (DTM). The results showed that the procedure for maintaini ng cultures in distilled water is simple, inexpensive and reliable.