Ri. Woodruff et Lg. Tilney, INTERCELLULAR BRIDGES BETWEEN EPITHELIAL-CELLS IN THE DROSOPHILA OVARIAN FOLLICLE - A POSSIBLE AID TO LOCALIZED SIGNALING, Developmental biology (Print), 200(1), 1998, pp. 82-91
In the epithelium of Drosophila ovarian follicles, cytoplasm-filled in
tercellular bridges connect epithelial cells. This study presents furt
her descriptive information about the morphology of these intercellula
r bridges and the extent of their distribution. We also offer speculat
ions concerning the possible developmental importance of the epithelia
l bridges. These bridges,,whose luminal diameters averaged 0.25 mu m,
are smaller than those forming the ring canals joining germline cells:
nor do they increase their size over time. The membranes limiting the
bridges are lined on the cytoplasmic side with an electron-dense mate
rial to which is attached a monolayer of filaments which encircle the
bridge. By decoration with the S1 fragment of myosin, these filaments
are confirmed as actin filaments. Following disruption of gap junction
al dye coupling by treatment with 1 mM octanol, microinjection of Luci
fer yellow CH revealed the extent and distribution of follicle cell in
tercellular bridges to be confined to arrays of no more than eight cel
ls/cluster, with many such independent clusters comprising the epithel
ium. Thus cell-to-cell movement throughout the epithelium of cytosolic
regulatory molecules cannot occur via these intercellular bridges. Ho
wever, weak signals affecting only one or a few cells in each cluster
would be amplified throughout the group by spread through the intercel
lular bridges. (C) 1998 Academic Press.