D. Griess et J. Rech, MICROSCOPIC CHARACTERIZATION OF CONSTITUENTS OF ANIMAL ORIGIN IN FEEDSTUFFS FOR ANIMALS, Revue de Medecine Veterinaire, 149(7), 1998, pp. 745-750
The Bovine Spongiform Encephalitis (BSE) and the prohibition of animal
meals in particular instances brought again the importance of the mic
roscopic diagnosis of the components in the mixed feeds. The presence
of animal meal is insured by the observation of specific constituents
: muscle fibers, bone fragments, feather particles, hair or wool bits,
fish scales. This observation is made by microscopy (magnification 10
0' up to 200'), after a fine pulverisation and a separation according
the specific gravimetry in carbon tetrachlor (CCl4) and then a light h
ydrolysis with a 0.2 p 100 KOK solution. The caracteristic constituent
s of the more used animal meals are described and illustraded. This is
an original, fast, qualitative and simple method, its sensitivity is
high since it proceeds from the observation of a few constituents only
.