A GENETIC-LINKAGE MAP OF LENTIL (LENS SP.) BASED ON RAPD AND AFLP MARKERS USING RECOMBINANT INBRED LINES

A genetic linkage map of Lens sp. was constructed with 177 markers (89 RAPD, 79 AFLP, six RFLP and three morphological markers) using 86 rec ombinant inbred lines (F-6:8) Obtained from a partially interspecific cross. The map covered 1073 cM of the lentil genome with an average di stance of 6.0 cM between adjacent markers. Previously mapped RFLP mark ers were used as anchor probes. The morphological markers, pod indehis cence, seed-coat pattern and flower-color loci were mapped. Out of the total linked loci, 8.4% showed segregation distortion. More than one- fourth of the distorted loci were clustered in one linkage group. AFLP markers showed more segregation distortion than the RAPD markers. The AFLP and RAPD markers were intermingled and clustering of AFLPs was s eldom observed. This is the most extensive genetic linkage map of lent il to-date. The marker density of this map could be used for the ident ification of markers linked to quantitative trait loci in this populat ion.