CLONING, QUANTIFICATION AND CHARACTERIZATION OF A MINISATELLITE DNA-SEQUENCE FROM COMMON BEAN PHASEOLUS-VULGARIS L

We describe the cloning and the characterization of a 130-bp DNA fragm ent, called OPG9-130, amplified from bean (Phaseolus vulgaris L.) geno mic DNA. This fragment corresponds to a minisatellite DNA sequence con taining seven repeats of 15 bp which differ slightly from each other i n their sequence. Southern analysis showed that the core sequence of 1 5 bp is repeated in clusters dispersed throughout the genome. The use of this fragment as a probe allowed us to identify common bean lines b y their DNA fingerprints. We suggest that OPG9-130 will be useful for line identification as well as for the analysis of genetic relatedness between bean species and lines.