RESPONSES OF RAPIDLY ADAPTING AFFERENT NEURONS TO DYNAMIC STRETCH OF RAT HAIRY SKIN

Twenty-four rapidly adapting (RA) cutaneous afferents were recorded fr om a preparation of isolated, innervated hairy skin from the rat hindl imb for the purpose of identifying the mechanical variables associated with the initiation of afferent discharge. Neurons were studied while the skin was stretched dynamically along a single direction with the use of a linear actuator and a feedback controller. Input signals were load- or displacement-controlled stretches that followed either perio dic or pseudorandom Gaussian noise control signals. When the tissue wa s actuated, loads and displacements were measured along the direction of stretch and neuronal responses were recorded. All RA afferents were activated by dynamic stretching. None had a sustained response to sta tic stretch. Cross-correlation products, calculated between neuronal r esponses and either stress- or strain- related variables observed at t he time of the spike, revealed a strong relationship between neuronal responses and tensile stress. Neuronal responses were observed at rate s of change of stress between +1,000 and -800 kPa/s. Neuronal response s were poorly related to skin strain. Two loading conditions were used along the direction transverse to the stretch. In one condition the s ides were unconstrained, so that on axial loading there was zero stres s and negative strain along the transverse axis. In the other conditio n the sides were constrained so that when the tissue was loaded axiall y there was zero strain and positive stress along the transverse axis. In these two conditions the same level of axial stress was associated with two levels of axial strain. The neuronal responses were determin ed by the stress and not the strain. Neuronal responses were observed at stresses >5 kPa. It appears that RA afferents make little contribut ion to signaling limb movements or position in rat hindlimb on the bas is of the behavior of rat hindlimb skin, as observed when the limb is rotated.