PH INFLUENCES GROWTH AND PLASMID STABILITY OF RECOMBINANT LACTOCOCCUS-LACTIS SUBSP LACTIS

Authors
BEAL C DANGIO C CORRIEU G
Citation
C. Beal et al., PH INFLUENCES GROWTH AND PLASMID STABILITY OF RECOMBINANT LACTOCOCCUS-LACTIS SUBSP LACTIS, Biotechnology letters, 20(7), 1998, pp. 679-682
Citations number
20
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
7
Year of publication
1998
Pages
679 - 682
Database
ISI
SICI code
0141-5492(1998)20:7<679:PIGAPS>2.0.ZU;2-X
Abstract
Continuous cultures of a recombinant Lactococcus lactis subsp. lactis strain were performed to display the effect of the fermentation pH on the specific growth rate and plasmid stability. The proportion of plas mid pIL252 bearing cells decreased exponentially with the number of ge nerations. The influence of the pH on the rate of loss of plasmid pIL2 52 and on the specific growth rate of L. lactis IL2682 was described b y second order polynomial equations. Optimal pH for the growth and pla smid stability were 6.39 and 6.41 respectively.