CONVERSION OF TRYPSIN INTO A SELENIUM-CONTAINING ENZYME BY USING CHEMICAL MUTATION

Authors
LIU JQ JIANG MS LUO GM YAN GL SHEN JC
Citation
Jq. Liu et al., CONVERSION OF TRYPSIN INTO A SELENIUM-CONTAINING ENZYME BY USING CHEMICAL MUTATION, Biotechnology letters, 20(7), 1998, pp. 693-696
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
7
Year of publication
1998
Pages
693 - 696
Database
ISI
SICI code
0141-5492(1998)20:7<693:COTIAS>2.0.ZU;2-6
Abstract
A chemical derivative of trypsin, selenotrypsin, was prepared to imita te glutathione peroxidase (GPX) by converting active serine residues i n the active site of trypsin into selenocysteines. The strategy for pr eparation of selenotrypsin contained selective sulfonation by phenylme thanesulfonyl fluoride and nucleophilic substitution by NaHSe. Selenot rypsin displayed enzyme activity of GPX, which is 150U/(mol. The kinet ic properties of selenotrypsin was demonstrated to be similar to that of native GPX.