PREPARATION OF HUMAN IMMORTALIZED B-CELLS SECRETING ANTIBODIES AGAINST FOOD ALLERGENS

Authors
SHINMOTO H NAKAHARA K KOBORI M TSUSHIDA T
Citation
H. Shinmoto et al., PREPARATION OF HUMAN IMMORTALIZED B-CELLS SECRETING ANTIBODIES AGAINST FOOD ALLERGENS, Biotechnology techniques, 12(7), 1998, pp. 545-547
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
7
Year of publication
1998
Pages
545 - 547
Database
ISI
SICI code
0951-208X(1998)12:7<545:POHIBS>2.0.ZU;2-G
Abstract
An immortalized B-cell library consisting of cells secreting antibodie s to food allergens has been prepared. Peripheral B-cells from seven h ealthy donors were transformed with Epstein-Barr virus and an immortal ized human B-cell library with 2,202 ampoules of multi-clone B-lymphob lastoid cells was obtained. The B-cell library contained various types of B-lymphoblastoid cells secreting antibodies to rice, soybeans, mil k, and eggs, and can be used to analyze food allergens.