RAPID METHOD FOR THE DETERMINATION OF LIPID FROM THE GREEN-ALGA BOTRYOCOCCUS-BRAUNII

Authors
Citation
Sj. Lee et al., RAPID METHOD FOR THE DETERMINATION OF LIPID FROM THE GREEN-ALGA BOTRYOCOCCUS-BRAUNII, Biotechnology techniques, 12(7), 1998, pp. 553-556
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
ISSN journal
0951208X
Volume
12
Issue
7
Year of publication
1998
Pages
553 - 556
Database
ISI
SICI code
0951-208X(1998)12:7<553:RMFTDO>2.0.ZU;2-Z
Abstract
Of various methods for lipid recovery in Botryococcus braunii UTEX 572 , the most effective method was disruption of the cells with a bead-be ater followed by extraction with chloroform:methanol (2:1, v/v). This gave a lipid content of 28.6% of dry wt. There was a significant relat ionship between in vivo fluorescence of cells stained with Nile Red an d lipid content in B. braunii determined gravimetrically (r(2) = 0.997 ). This suggested that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination w hich requires toxic solvents and considerable time-consuming manipulat ions.