Sj. Lee et al., RAPID METHOD FOR THE DETERMINATION OF LIPID FROM THE GREEN-ALGA BOTRYOCOCCUS-BRAUNII, Biotechnology techniques, 12(7), 1998, pp. 553-556
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Of various methods for lipid recovery in Botryococcus braunii UTEX 572
, the most effective method was disruption of the cells with a bead-be
ater followed by extraction with chloroform:methanol (2:1, v/v). This
gave a lipid content of 28.6% of dry wt. There was a significant relat
ionship between in vivo fluorescence of cells stained with Nile Red an
d lipid content in B. braunii determined gravimetrically (r(2) = 0.997
). This suggested that the Nile Red staining as a rapid method was as
good as the gravimetric method commonly used for lipid determination w
hich requires toxic solvents and considerable time-consuming manipulat
ions.