PECTINMETHYLESTERASE INACTIVATION BY BUBBLING WITH N-2

Authors
CAUSSETTE M GAUNAND A PLANCHE H COLOMBIE S MONSAN P LINDET B
Citation
M. Caussette et al., PECTINMETHYLESTERASE INACTIVATION BY BUBBLING WITH N-2, Biotechnology techniques, 12(7), 1998, pp. 561-564
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
7
Year of publication
1998
Pages
561 - 564
Database
ISI
SICI code
0951-208X(1998)12:7<561:PIBBWN>2.0.ZU;2-G
Abstract
The half-life of pectinmethylesterase decreased from 8.5 h in an aqueo us solution to 3.5 h when 100 mL/N-2 min(-1) was bubbled through it at 50 degrees C. Inactivation strongly depended on the pH and the ionic strength and was enhanced at a pH close to the pI of the enzyme.