RAPID PREPARATION IN HIGH-YIELD OF PURE FORMATE DEHYDROGENASE FROM PICHIA-AUGUSTA

Authors
NEUHAUSER W STEININGER M HALTRICH D KULBE KD NIDETZKY B
Citation
W. Neuhauser et al., RAPID PREPARATION IN HIGH-YIELD OF PURE FORMATE DEHYDROGENASE FROM PICHIA-AUGUSTA, Biotechnology techniques, 12(7), 1998, pp. 565-568
Citations number
16
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
7
Year of publication
1998
Pages
565 - 568
Database
ISI
SICI code
0951-208X(1998)12:7<565:RPIHOP>2.0.ZU;2-3
Abstract
Biomimetic dye ligand chromatography and reversible ionic strength-dep endent protein precipitation enabled isolation of formate dehydrogenas e from the yeast Pichia angusta in purity of >95%. The enzyme has a sp ecific NAD-linked activity of 5.7 units/mg and was obtained in stable form, yield of 88% and concentration of about 20 mg/ml.