STABILITY OF ENZYMES IN WATER ORGANIC COSOLVENT MIXTURES/

Authors
TYAGI R GUPTA MN
Citation
R. Tyagi et Mn. Gupta, STABILITY OF ENZYMES IN WATER ORGANIC COSOLVENT MIXTURES/, Biotechnology techniques, 12(7), 1998, pp. 569-570
Citations number
11
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
7
Year of publication
1998
Pages
569 - 570
Database
ISI
SICI code
0951-208X(1998)12:7<569:SOEIWO>2.0.ZU;2-Y
Abstract
The half lives of polyphenol oxidase and trypsin at 50% (v/v) co-solve nt (water miscible) were from 23 to 176 h when polarity index of the c o-solvent was >5.8 or if the cc-solvent was an alcohol. Polyphenol oxi dase, trypsin, chymotrypsin and beta-glucosidase lost only 10% activit y in such water/organic co-solvent mixtures when the organic co-solven ts were present in the concentration ranges of 55-90%, 60-78%, 15-70% and 7-25% respectively.