DETECTION OF ESCHERICHIA-COLI O157-H7 USING A SURFACE-PLASMON RESONANCE BIOSENSOR

The BIAcore biosensor was used to detect binding of Escherichia coli O 157:H7 with specific antibodies. Immobilized Protein A or Protein G ca ptured antibodies which in turn bound to the bacteria. Alternatively, immobilized antibody captured the E. coli O157:H7 and the bacteria wer e further probed by a second antibody which enhanced the signal. The r egenerated sensor surfaces were used for at least 50 separate analyses . The surface plasmon resonance biosensor has potential for use in rap id, real-time detection and identification of bacteria, and to study t he interaction of organisms with different antisera or other molecular species.