DEVELOPMENT OF A MAGNETIC MICROPLATE CHEMIFLUORIMMUNOASSAY FOR RAPID DETECTION OF BACTERIA AND TOXIN IN BLOOD

Authors
YU H AHMED H VASTA GR
Citation
H. Yu et al., DEVELOPMENT OF A MAGNETIC MICROPLATE CHEMIFLUORIMMUNOASSAY FOR RAPID DETECTION OF BACTERIA AND TOXIN IN BLOOD, Analytical biochemistry (Print), 261(1), 1998, pp. 1-7
Citations number
14
Categorie Soggetti
Biology,"Biochemical Research Methods","Chemistry Analytical
Journal title
Analytical biochemistry (Print) → ACNP
ISSN journal
00032697
Volume
261
Issue
1
Year of publication
1998
Pages
1 - 7
Database
ISI
SICI code
0003-2697(1998)261:1<1:DOAMMC>2.0.ZU;2-L
Abstract
A magnetic microplate chemifluorimmunoassay (MMCLA) is described using an immunomagnetic separation and a fluorescent microplate technique f or rapid detection of low-level Escherichia coli O157:H7, Bacillus sub tilis var. niger spores, and Staphylococcal enterotoxin type B from wh ole blood, In general, the MMCIA has at least several-fold more sensit ivity than the conventional enzyme-linked immunosorbent assay. In addi tion, the assay sensitivities using direct fluorochrome label as the r eporter, or alkaline phosphatase (AP) with various assay substrates, s uch as pNPP and AttoPhos, were assessed. (C) 1998 Academic Press.