DETERMINATION OF THE FRACTION COMPOSITION OF BLOOD LIPOPROTEINS BY THE SMALL-ANGLE X-RAY-SCATTERING TECHNIQUE

A new method for analysis of the fraction composition (FC) of blood li poproteins (LP) was developed based on the small-angle X-ray scatterin g technique (SAXS). The method allows quantitative determination of th e contents of basic LP fractions (high density LP, low density LP, ver y low density LP and their subfractions) in the blood plasma or serum. The results of LP analysis by the new method were compared to the ele ctron microscopy, ultracentrifugation and gel electrophoresis data. Th e results obtained by the SAXS method correlated with those obtained b y traditional methods. The new method for the determination of the LP FC in the blood is rapid (1-1,5 hour), uses only one reagent (e.g. suc rose) and is characterized by the high accuracy and resolution up to L P subfractions. A total of the 0,05 ml of the blood plasma or serum is required for analysis. The analysis can be carried out in the purifie d preparations and in the blood plasma or serum. The method developed can be used in clinical practice for diagnostics and in scientific res earch.