J. Tallkvist et H. Tjalve, TRANSPORT OF NICKEL ACROSS MONOLAYERS OF HUMAN INTESTINAL CACO-2 CELLS, Toxicology and applied pharmacology, 151(1), 1998, pp. 117-122
The passage of nickel across monolayers of intestinal epithelial Caco-
2 cells, originally derived from a human colonic adenocarcinoma, was s
tudied in bicameral chambers. The results showed that the transport an
d accumulation of nickel were depressed in iron-loaded monolayers, ind
icating that the metal participates in an absorptive process for iron
in the Caco-2 cells. No detectable transport of nickel in either the a
pical to basal or basal to apical direction occurred at 4 degrees C. S
ince cellular metabolism is inhibited at 4 degrees C, these data indic
ate that there is no passive transcellular or paracellular passage of
the nickel across the monolayers. Studies in ATP-depleted monolayers s
howed an increased permeability of nickel, and concomitantly there was
a similar increase in the permeability of the paracellular marker man
nitol, These results indicate that the metabolic inhibition results in
a loosening of the junctional complexes between the Caco-2 cells, res
ulting in a paracellular leakage of the nickel. Additional experiments
showed that the transport of nickel in the basal to apical direction
occurred at a higher rate than in the apical to basal direction, This
indicates the presence of an extrusion mechanism that secretes the nic
kel from the basal to the apical side of the Caco-2 cells. Studies wit
h Caco-2 cells and in vivo studies by other authors have shown similar
results for other metals, indicating that colonic epithelial cells ma
y have the ability to secrete some metals, (C) 1998 Academic Press.