Cm. Gomes et M. Teixeira, AMBINEELA, AN UNUSUAL BLUE PROTEIN ISOLATED FROM THE ARCHAEON ACIDIANUS-AMBIVALENS, Biochemical and biophysical research communications (Print), 249(1), 1998, pp. 23-25
A novel blue protein, named ambineela, was isolated from the soluble e
xtract of the thermoacidophilic archaeon Acidianus ambivalens. In solu
tion, the purified protein is a monomer with 50 kDa and has a basic ch
aracter (pI similar to 8.7). The electronic spectrum shows two bands,
centred at 395 and 625 nm (A(625)/A(395) = 0.7). The protein does not
contain any transition metal; its blue colour is due to an unidentifie
d non-fluorescent cofactor, covalently bound to it. Ambineela N-termin
al sequence exhibits a consensus ADP-binding region, suggesting that i
ts unknown cofactor may comprise this molecule or an analogue. (C) 199
8 Academic Press.