A SIMPLE METHOD FOR SEQUENCING SMALL DNAS BY INTRODUCING PRECISE OVERLAPPING ENDS INTO RESTRICTION DIGESTION FRAGMENTS

A method has been devised whereby certain complete restriction digesti ons can have short overlaps of unique sequence incorporated into the f ragments during cloning. Thus one can identify when the DNA fragments are contiguous. Here, this technique is used to produce a contig for a 2.5 kb fragment of genomic DNA. This is a simple approach to ordering digestion fragments without necessarily performing restriction mappin g. It is envisaged that this technique will be useful for sequencing c DNAs and small genomic fragments.