A RAPID AND SENSITIVE ASSAY FOR HISTONE ACETYL-TRANSFERASE ACTIVITY

Authors
AITSIALI S RAMIREZ S ROBIN P TROUCHE D HARELBELLAN A
Citation
S. Aitsiali et al., A RAPID AND SENSITIVE ASSAY FOR HISTONE ACETYL-TRANSFERASE ACTIVITY, Nucleic acids research, 26(16), 1998, pp. 3869-3870
Citations number
14
Categorie Soggetti
Biology
Journal title
Nucleic acids research → ACNP
ISSN journal
03051048
Volume
26
Issue
16
Year of publication
1998
Pages
3869 - 3870
Database
ISI
SICI code
0305-1048(1998)26:16<3869:ARASAF>2.0.ZU;2-A
Abstract
Histone acetyl-transferases (HATs) seem to be key elements in the regu lation of transcription. We have designed an enzymatic assay to quanti fy HAT enzymatic activity. In this assay, the substrate is a peptide c orresponding to the 24 first amino acids of histone H4 which is couple d to biotin. After acetylation using [C-14]acetyl-CoA, the peptide is purified on streptavidin beads and the associated radioactivity is mea sured. This assay is sensitive, rapid and convenient.