ORIENTATION AND ROTATIONAL-DYNAMICS OF SPIN-LABELED PHALLOIDIN BOUND TO ACTIN IN MUSCLE-FIBERS

We have used electron paramagnetic resonance spectroscopy (EPR) to inv estigate the orientational distribution of actin in thin filaments of glycerinated muscle fibers in rigor, relaxation, and contraction. A sp in-labeled derivative of a mushroom toxin, phalloidin (PHSL), was boun d to actin in the muscle fibers (PHSL-fibers). The EPR spectrum of uno riented PHSL-labeled myofibrils consisted of three sharp lines with a splitting between the outer extrema (2T(parallel-to)') of 42.8 +/- 0.1 G, indicating that the spin labels undergo restricted nanosecond rota tional motion within an estimated half-cone angle of 76-degrees. When the PHSI-fiber bundle was oriented parallel to the magnetic field, the splitting between the zero-crossing points (2T') was 42.7 +/- 0.1 G. When the fiber bundle was perpendicular to the magnetic field, 2T' dec reased to 34.5 +/- 0.2 G. This anisotropy shows that the motion of the probe is restricted in orientation by its binding site on actin, so t hat the EPR spectrum of PHSL-fiber bundles would be sensitive to small changes in the mean axial orientation of the PHSL-actin interface. No differences in the EPR spectra were observed in fibers during rigor, relaxation, or contraction, indicating that the mean axial orientation of the PHSL binding site changes by less than 5-degrees, and that the amplitude of nanosecond probe rotational motion, which should be quit e sensitive to the local environment of the phalloidin, changes by no more than 1-degrees. These results rule out large changes in the overa ll geometry of the actin filament and in the local conformation of act in near the phalloidin binding site during the generation of isometric tension in muscle fibers. (C) 1993 Wiley-Liss, Inc.