CRYSTALLIZATION AND PRELIMINARY-X-RAY ANALYSIS OF NONGLYCOSYLATED TISSUE INHIBITOR OF METALLOPROTEINASES-1, N30QN78Q TIMP-1

A nonglycosylated (N30QN78Q) form of the human tissue inhibitor of met alloproteinases, TIMP-1, has been prepared and crystallized in a form suitable for X-ray diffraction analysis. Small single crystals have be en grown using sodium tartrate as a precipitant. The crystals are in s pace group P2(1), with cell dimensions a = 35.28, b = 53.95, c = 48.56 , and beta = 96.0-degrees. There is a single molecule of TIMP-1 in the asymmetric unit. The crystals diffract to at least 2.3 angstrom resol ution. Complete data have been collected to 2.9 angstrom and a search for heavy-metal derivatives is in progress. (C) 1993 Wiley-Liss, Inc.