AN APPRAISAL OF THE USE OF RECIPROCAL TRANSFER EXPERIMENTS - ASSESSING THE STAGES OF PHOTOPERIOD SENSITIVITY IN CHRYSANTHEMUM CV SNOWDON (CHRYSANTHEMUM-MORIFOLIUM RAMAT)
Sr. Adams et al., AN APPRAISAL OF THE USE OF RECIPROCAL TRANSFER EXPERIMENTS - ASSESSING THE STAGES OF PHOTOPERIOD SENSITIVITY IN CHRYSANTHEMUM CV SNOWDON (CHRYSANTHEMUM-MORIFOLIUM RAMAT), Journal of Experimental Botany, 49(325), 1998, pp. 1405-1411
Reciprocal transfer experiments can be used to describe the stages of
photoperiod sensitivity in daylength-sensitive plants. However, there
are inconsistencies in the literature concerning the techniques used a
nd, more importantly, the assumptions made when analysing such data se
ts. This paper appraises the use of reciprocal transfer experiments, w
ith chrysanthemum as a model (short day) plant. Experiments showed lit
tle evidence to suggest that axillary meristems were incapable of resp
onding to a floral stimulus when released from apical dominance by pin
ching (even though the apex appeared vegetative). Five short days give
n after pinching resulted in sufficient induction to initiate an inflo
rescence, although seven short days were required to commit a plant to
flower with a leaf number similar to plants grown in continuous short
days. Floral initiation was then visible at the apex after nine short
days, Once the inflorescence had been initiated, long days delayed th
e early stages of flower development. The results are discussed with r
eference to reciprocal transfer experiments in general, and specifical
ly in relation to problems that arise when the length of a 'juvenile'
phase is confounded with the number of inductive cycles for flower com
mitment.