A pollution event which occurred at a spring in the Arnside area of Cu
mbria provided an opportunity to investigate whether Campylobacter jej
uni could be detected in groundwater. Hydrological evidence suggested
that the source of contamination was a dairy farm situated within the
hydrological catchment of the polluted spring. The microbiological qua
lity of the polluted spring was monitored during intervals over the fo
llowing 12 months and compared with others in the area. Campylobacter
Jejuni was isolated by filter enrichment of 500 mi and 100 mi filtered
volumes of groundwater. It was not isolated in the absence of faecal
indicator species. Some strains of Camp. jejuni from water had identic
al biotypes to strains isolated from the dairy herd. This paper report
s the first isolation of Camp. jejuni from groundwater using cultural
methods and supports the theory that groundwater may be a vehicle for
Campylobacter transmission.