Dj. Wolyn et A. Chahal, NUCLEAR AND CYTOPLASMIC INTERACTIONS FOR PETALOID MALE-STERILE ACCESSIONS OF WILD CARROT (DAUCUS-CAROTA L.), Journal of the American Society for Horticultural Science, 123(5), 1998, pp. 849-853
Male-sterile carrot plants with petaloid-stamens were discovered in wi
ld populations in the vicinity of Guelph, Ontario, Canada and inherita
nce of phenotype was assessed. Genetic analyses were also conducted to
identify nuclear restorer genes of petaloid cytoplasmic male sterilit
y (CMS) from wild carrot, and determine if Guelph, Cornell and Wiscons
in cytoplasmic male-sterile accessions interacted similarly with allel
es of restorer genes from different sources. Petaloid male-sterility w
as inherited cytoplasmically for the new Guelph accessions, and nuclea
r genotypes which normally produce 'green' or 'white' petaloid-stamens
in combination with the Cornell cytoplasm also produced corresponding
phenotypes when backcrossed to Guelph cytoplasmic accessions. Two dup
licate restorer genes were identified from wild carrot. Dominant allel
es of single restorer genes from three sources, one from wild carrot a
nd two from cultivated germplasm, restored stamen production to Guelph
, Cornell and Wisconsin petaloid cytoplasmic accessions, suggesting th
at the cytoplasms were genetically identical.