The equine laminar junction plays a vital role in transferring the for
ces of weight-bearing between the epidermal hoof wall and the bone of
the third phalanx, but the way in which it performs this function is p
oorly understood, Using samples from sites varying proximodistally and
circumferentially around the hoof, the stress/strain behaviour of thi
s tissue was characterised in three directions: radial tension and pro
ximodistal and mediolateral shear, The influences of toe angle and len
gth were also examined. For all three test directions, the modulus of
elasticity increased with increasing strain magnitude. The mean modulu
s of elasticity in tension was 18.25 +/- 5.38 MPa (mean +/- 1 S.D., N=
116; mean strain 0.25), In proximodistal shear, the mean shear modulus
was 5.38 +/- 1.49 MPa (N=76; mean shear strain 0.48) and in mediolate
ral shear 2.57 +/- 0.91 MPa (N=66: mean shear strain 0.81), In many ca
ses, the individual hoof or horse from which the samples were taken si
gnificantly affected the value of the modulus, suggesting that mechani
cal history may affect the material properties of this tissue. Few bio
logically significant variations with location, toe length or toe angl
e were unambiguously demonstrated, suggesting that the material proper
ties of the laminar junction are independent of position, despite appa
rent regional variations in function, and that foot shape is not a maj
or determinant of material properties.