A ROLE FOR CD5 IN COGNATE INTERACTIONS BETWEEN T-CELLS AND B-CELLS, AND IDENTIFICATION OF A NOVEL LIGAND FOR CD5

CD5 is a glycoprotein expressed at a high level on the surface of matu re T lymphocytes. Studies with CD5 mAb and CD5-deficient mice have sho wn that the CD5 molecules have a significant role in T cell growth res ponse. However, the precise role of CD5 in immune cell interactions is still unclear. The present study provides evidence that CD5 plays a d irect role in providing growth signals during the contact-dependent ac tivation and proliferation of splenic a cells. An anti-CDS mAb inhibit ed T(h)1- and T(h)2-type cell-induced a cell proliferation. CD5-Ig, a chimeric fusion protein, induced proliferation of resting a cells. Flo w cytometric analyses using CD5-Ig and mAb to CD72 demonstrated that C D5 bound to a ligand (CD5L), and this binding was not blocked by a var iety of anti-CD72 mAb, Also, CD5-Ig did not bind to CD72(+)-transfecte d cells. Immunoprecipitation of surface labeled a cell molecules with CD5-Ig showed that CD5L was composed of 77-80 and 38-40 kDa polypeptid e chains, distinct from CD72, CD5L was expressed on activated splenic a cells, but not T cells, whereas its expression was constitutive on p eritoneal a cells and on a lymphoma cell lines.