NOVEL METHOD FOR RAPID MEASUREMENT OF GROWTH OF MYCOBACTERIA IN DETERGENT-FREE MEDIA

Authors
MEYERS PR BOURN WR STEYN LM VANHELDEN P BEYERS AD BROWN GD
Citation
Pr. Meyers et al., NOVEL METHOD FOR RAPID MEASUREMENT OF GROWTH OF MYCOBACTERIA IN DETERGENT-FREE MEDIA, Journal of clinical microbiology, 36(9), 1998, pp. 2752-2754
Citations number
16
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
9
Year of publication
1998
Pages
2752 - 2754
Database
ISI
SICI code
0095-1137(1998)36:9<2752:NMFRMO>2.0.ZU;2-6
Abstract
We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Myc obacterium smegmatis in the presence or absence of detergent. The meth od, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favorably with other methods for mon itoring mycobacterial growth but is particularly useful for heavily cl umped cultures grown in defined minimal medium.