DEVELOPMENT OF SPECIFIC NESTED OLIGONUCLEOTIDE PCR PRIMERS FOR THE STREPTOCOCCUS-INIAE 16S-23S RIBOSOMAL DNA INTERGENIC SPACER

Authors
BERRIDGE BR FULLER JD DEAZAVEDO J LOW DE BERCOVIER H FRELIER PF
Citation
Br. Berridge et al., DEVELOPMENT OF SPECIFIC NESTED OLIGONUCLEOTIDE PCR PRIMERS FOR THE STREPTOCOCCUS-INIAE 16S-23S RIBOSOMAL DNA INTERGENIC SPACER, Journal of clinical microbiology, 36(9), 1998, pp. 2778-2781
Citations number
22
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
36
Issue
9
Year of publication
1998
Pages
2778 - 2781
Database
ISI
SICI code
0095-1137(1998)36:9<2778:DOSNOP>2.0.ZU;2-T
Abstract
Streptococcus iniae is a cause of septicemia, meningoencephalitis, and death in farmed fish and of cellulitis in human beings. A set of nest ed oligonucleotide PCR primers that specifically amplified a 373-bp su bunit from a variety of clinical isolates from farmed fish and human p atients were constructed from a 524-bp consensus sequence of the S. in iae 16S-23S ribosomal DNA intergenic spacer.