A MAMMALIAN 2-PORE DOMAIN MECHANO-GATED S-LIKE K+ CHANNEL

Aplysia S-type K+ channels of sensory neurons play a dominant role in presynaptic facilitation and behavioural sensitization. They are close d by serotonin via cAMP-dependent phosphorylation, whereas they are op ened by arachidonic acid, volatile general anaesthetics and mechanical stimulation. We have identified a cloned mammalian two P domain K+ ch annel sharing the properties of the S channel. In addition, the recomb inant channel is opened by lipid bilayer amphipathic crenators, while it is closed by cup-formers. The cytoplasmic C-terminus contains a cha rged region critical for chemical and mechanical activation, as well a s a phosphorylation site required for cAMP inhibition.