ELECTROCHEMICAL PROBE FOR POLYAMINES DETECTION IN BIOLOGICAL-FLUIDS

The enzyme polyamine oxidase from maize has been purified and covalent ly immobilized onto polymeric supports to assemble a polyamine electro chemical biosensor. The enzyme has been used in conjunction with eithe r an O-2 or a H2O2 electrode. The analytical behaviour of this enzyme electrode has been studied with respect to sensitivity, selectivity, o ptimum pH and buffer, response time and lifetime. Recovery studies per formed on urine samples demonstrated that the polyamine biosensor deve loped can be successfully used to measure polyamines in biological flu ids.