REGENERATIVE RESPONSE IN ACUTE-RENAL-FAILURE DUE TO VITAMIN-E-DEFICIENCY AND GLUTATHIONE DEPLETION IN RATS

In this study, we investigated some factors contributing to renal rege neration after acute renal failure (ARF) induced by vitamin E (VE) def iciency and glutathione (GSH) depletion. Acute renal failure was induc ed by feeding rats a vitamin E-deficient diet for 6 weeks and then inj ecting buthionine sulfoximine (BSO), a glutathione-depleting agent. Th e level of hepatocyte growth factor (HGF), a renotropic factor for reg eneration in the kidney, showed a transient increase at 5 hr after the BSO treatment. Subsequently, renal ornithine decarboxylase (ODC) acti vity, a marker of G(1) phase, and labeling index (LI) of proliferating cell nuclear antigen (PCNA), a marker of DNA synthesis (S phase), rea ched peaks at 10 and 53 hr after the injection, respectively. Thus, it appears that the increase in ornithine decarboxylase activity and sub sequent elevation in proliferating cell nuclear antigen labeling index following the increase in the hepatocyte growth factor level in the k idneys are closely related to the renal regenerative response after ac ute renal failure. BIOCHEM PHARMACOL 56;4: 543-546, 1998. (C) 1998 Els evier Science Inc.