RAPID AND EFFECTIVE PROCESSING OF BLOOD SPECIMENS FOR DIAGNOSTIC PCR USING FILTER-PAPER AND CHELEX-100

Aim-To provide a more efficient method for isolating DNA from peripher al blood for use in diagnostic DNA mutation analysis. Methods-The use of blood impregnated filter paper and Chelex-100 in DNA isolation was evaluated and compared with standard DNA isolation techniques. Results -In polymerase chain reaction (PCR) based assays of five point mutatio ns, identical results were obtained with DNA isolated routinely from p eripheral blood and isolated using the filter paper and Chelex-100 met hod. Conclusion-In the clinical setting, this method provides a useful alternative to conventional DNA isolation. It is easily implemented a nd inexpensive, and provides sufficient, stable DNA for multiple assay s. The potential for specimen contamination is reduced because most of the steps are performed in a single microcentrifuge tube. In addition , this method provides for easy storage and transport of samples from the paint of acquisition.