COMPETITIVE-BINDING ASSAY FOR THYROXINE USING IN-VITRO SELECTED OLIGONUCLEOTIDES

Authors
ITO Y FUJITA S KAWAZOE N IMANISHI Y
Citation
Y. Ito et al., COMPETITIVE-BINDING ASSAY FOR THYROXINE USING IN-VITRO SELECTED OLIGONUCLEOTIDES, Analytical chemistry (Washington), 70(16), 1998, pp. 3510-3512
Citations number
10
Categorie Soggetti
Chemistry Analytical
Journal title
Analytical chemistry (Washington) → ACNP
ISSN journal
00032700
Volume
70
Issue
16
Year of publication
1998
Pages
3510 - 3512
Database
ISI
SICI code
0003-2700(1998)70:16<3510:CAFTUI>2.0.ZU;2-S
Abstract
A new binding assay that uses oligodeoxyribonucleotides (DNAs) obtaine d by the in vitro selection method, instead of antibodies, to bind to the target molecule, thyroxine (T-4), is described, The DNAs which sel ectively bound to the T-4 were selected, labeled with biotin or radioi sotope, and then used to detection of T-4 in the presence of liothyron ine (T-3), which has a chemical structure similar to that of T-4.