DETERMINATION OF LANSOPRAZOLE IN BIOLOGICAL-FLUIDS AND PHARMACEUTICALDOSAGE BY HPLC

A simple and rapid (extractionless) high-performance liquid chromatogr aphic method with UV detection at 230 nm was developed for the determi nation of lansoprazole in biological fluids and pharmaceutical dosage. Niflumic acid was added as internal standard. The separation was perf ormed at ambient temperature on a C-18 Spherisorb column with acetonit rile + 0.1 M sodium acetate (40:60, v/v, pH 7) as mobile phase. The re tention time was 5.2 min for niflumic acid and 6.7 min for lansoprazol e. The detection limit was 20 ng/ml using a 100 mu l loop. The method was successfully applied to a pharmacokinetic study of lansoprazole in humans.