SUBCUTANEOUS NAAS3-RNA AND PROTEIN EXPRESSION IN JUVENILE WINTER FLOUNDER( EXPOSURE INCREASES METALLOTHIONEIN MESSENGER)

We investigated the effect of NaAs3+ exposure on liver metallothionein (MT) mRNA and protein expression in juvenile winter flounder (Pleuron ectes americanus). Sexually immature flounder (age 18 months) were sub cutaneously implanted with silastic tubing containing 0 (vector contro l), 1.5, 3.0, 6.0 and 16.0 mu mol g(-1) fish wt. NaAs3+. Liver MT mRNA and protein were significantly elevated within 24 h of exposure at th e 1.5 mu mol g(-1) dose before there was significant liver As accumula tion or mortality. All four NaAs3+ treatments caused a significant ris e in MT mRNA and protein and the mRNA demonstrated the dearest dose re sponse and greatest fold change (19 fold at 16.0 mu mol g(-1) dose). M T protein reached its maximum (1.8 times the control) at the 1.5 mu mo l g(-1) dose. This maximum was maintained across all four treatments a nd decreased slightly at the 16.0 mu mol g(-1) dose where there was 40 % mortality. The level of liver As content among fish with maximal MT expression was similar to that of fish which recently succumbed to NaA s3+ toxicity. This links saturation of the MT response with mortality and provides a framework for assessing the significance of MT measurem ents in feral flounder. The experiment was repeated (minus the 16.0 mu mol g(-1) dose) in a smaller (and younger) group of fish (12 months o ld) which expressed a two-fold lower liver Zn and MT protein content. This relatively low level of pre-existing MT theoretically widened the MT response window such that the MT maximum was not attained until th e 6.0 mu mol g(-1) dose. These fish also did not show a significant ri se in either MT mRNA or protein at the sublethal dose (1.5 mu mol g(-1 )). This lack of change appeared to reflect the higher level of baseli ne liver As which also did not increase at the 1.5 mu mol g(-1) dose. Reevaluation of the earlier experiment revealed a 1.5 fold increase in liver As content at the 1.5 mu mol g(-1) dose that was not significan t due to high variance. This suggests that MT is a better biomarker of NaAs3+ exposure because there is less variance associated with either MT mRNA or protein measurements. These results lend merit to MTOs use fulness as a biomarker of metal exposure and provides the foundation f or linking NaAs3+ exposure with MT expression and possibly metal-induc ed carcinogenesis in fish. (C) 1998 Elsevier Science B.V. All rights r eserved.