G. Mustafa et al., SEPARATION OF MOTILE SPERMATOZOA FROM FROZEN-THAWED BUFFALO SEMEN - SWIM-UP VS FILTRATION PROCEDURES, Theriogenology, 50(2), 1998, pp. 205-211
Three experiments were conducted to maximize the recovery rate of moti
le spermatozoa from frozen-thawed buffalo semen. In Experiment 1, the
swim-up of motile spermatozoa was performed in the presence or absence
of HEPES in TALP medium and CO2 in the environment. The recovery rate
of motile spermatozoa in TALP medium (control), TALP+HEPES+CO2, TALPHEPES and TALP+CO2 was 15, 18, 12 and 10%, respectively (P>0.05), with
sperm motility at 87 89, 90 and 90%, respectively (P>0.05). In Experi
ment 2 the pH of TALP medium was adjusted to 7.0, 7.5, 8.0, 8.5 and 9.
0, and swim-up procedure was performed in the presence of HEPES and CO
2. The recovery rate of motile spermatozoa at different pH was 14, 20,
24, 27 and 16%, respectively (P<0.05). Motility of separated spermato
zoa was 88, 91, 90, 89 and 90%, respectively (P>0.05). In Experiment 3
, the efficiency of ion-exchange filtration and Swim-up procedure in s
eparating motile spermatozoa from frozen-thawed buffalo semen was comp
ared. The recovery rate of motile spermatozoa was 95% in filtration pr
ocedure and 33% in swim-up procedure (P<0.005). In all experiments, no
rmal acrosomes did not vary due to treatments (P>0.05). In conclusion,
HEPES and CO:! had no significant effect on swim-up of buffalo sperma
tozoa. The pH 8.5 of TALP improved the recovery rate of motile spermat
ozoa in swim-up procedure. The ion-exchange filtration was found super
ior to swim-up procedure in harvesting maximum number of motile sperma
tozoa from frozen-thawed buffalo semen (95 vs 33%; P<0.001). (C) 1998
by Elsevier Science Inc.