CHEMOENZYMIC SYNTHESIS OF BETA-D-GAL(6-SO4)-(1-]4)-D-GLCNAC, BETA-D-GAL-(1-]4)-D-GLCNAC(6-SO4)AND BETA-D-GLCNAC-(1-]4)-D-GLCNAC(6-SO4) AND THEIR ROLES AS FUCOSYL ACCEPTORS IN REACTIONS CATALYZED BY HUMAN ALPHA-3-FUCOSYL-TRANSFERASES

Authors
TRAN CH CRITCHLEY P CROUT DHG BRITTEN CJ WITHAM SJ BIRD MI
Citation
Ch. Tran et al., CHEMOENZYMIC SYNTHESIS OF BETA-D-GAL(6-SO4)-(1-]4)-D-GLCNAC, BETA-D-GAL-(1-]4)-D-GLCNAC(6-SO4)AND BETA-D-GLCNAC-(1-]4)-D-GLCNAC(6-SO4) AND THEIR ROLES AS FUCOSYL ACCEPTORS IN REACTIONS CATALYZED BY HUMAN ALPHA-3-FUCOSYL-TRANSFERASES, Journal of the Chemical Society. Perkin transactions. I (Print), (15), 1998, pp. 2295-2299
Citations number
35
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Journal of the Chemical Society. Perkin transactions. I (Print) → ACNP
ISSN journal
0300922X
Issue
15
Year of publication
1998
Pages
2295 - 2299
Database
ISI
SICI code
0300-922X(1998):15<2295:CSOBB>2.0.ZU;2-A
Abstract
N-Acetyllactosamine 5 is obtained by transglycoslyation between p-nitr ophenyl beta-D-galactopyranoside 3 and N-acetyl-D-glucosamine 4 by usi ng the beta-galactosidase from Bacillus circulans as the biocatalyst, Sodium salts of beta-Gal(6-SO4)-(1-->4)-GlcNAc 1 and beta-Gal-(1-->4)- GlcNAc(6-SO4) 2 have been prepared from N-acetyllactosamine 5 in five and three steps respectively. beta-D-GlcNAc-(1-->4)-D-GlcNAc(6-SO4) (d i-N-acetylchitobiose 6-sulfate) 10 is synthesised from chitin in three steps. The products have been tested as fucosyl accepters in reaction s catalysed by recombinant human fucosyltransferases III to VII.