Electrical conductance measurements are being used experimentally for
occlusal caries detection. Recently, it was suggested to cover the fis
sure system with a conducting medium, resulting in a surface-specific
measurement. It was the aim of this study to determine in vitro the re
producibility of this modified technique for occlusal caries in poster
ior teeth, to determine for a large study sample the correlation betwe
en the electrical measurements and histological lesion depth, and to e
valuate the difference between results for premolars and molars, For t
he reproducibility determination, surface-specific electrical resistan
ce measurements were made using a sample of 68 posterior teeth. Eight
operators performed measurements on all teeth, and repeated measuremen
ts on 24 teeth. The validity study included the previous sample and th
e collected samples from two more studies, resulting in a total sample
of 325 posterior teeth. One operator had performed electrical resista
nce measurements on all teeth in the sample. Reproducibility was good:
mean Pearson's correlation coefficient 0.89 (+/-0.05) for interexamin
er correlation, and 0.86 (+/-0.12) for intra-examiner correlation, usi
ng log (resistance) as the result parameter. The correlation coefficie
nt between log(resistance) and histological lesion depth for the large
sample was -0.78 for all teeth, -0.64 for premolars, and -0.73 for mo
lars. The regression line for molars was located below the regression
line for premolars: at a hypothetical histology score of 2.5 (a dentin
e caries threshold) the estimated resistance threshold would be 507 k
Ohm for premolars, and 233 k Ohm for molars. Converted to Electronic C
aries Monitor (ECM) readings, the difference is about 1.4 on the ECM s
cale. It was concluded that the in vitro reproducibility of the descri
bed surface-specific method for electrical resistance measurement is v
ery good, even for inexperienced operators. The correlation between me
asurements and histological lesion depth is moderate to good, The meth
od is sensitive to electrode area differences, which will result in di
fferent clinical cut-offs for caries diagnosis in premolar and molar t
eeth.