INHIBITORY EFFECTS OF ANGELICA-PUBESCENS F BISERRATA ON 5-LIPOXYGENASE AND CYCLOOXYGENASE

Linoleic acid, osthol, osthenol and two polyacetylenes, falcarindiol a nd ),16(R)-dihydroxyoctadeca-9Z,17-diene-12,14-diyn-1 -yl acetate were found to be the most active compounds responsible for the inhibitory activity of the dichloromethane extract of the roots of Angelica pubes cens f. biserrata on 5-lipoxygenase (5-LO) and cyclooxygenase (COX-1) in vitro. They showed prominent inhibitory effect on 5-LO with IC50 va lues of 27.9 mu M, 36.2 mu M, 43.1 mu M, 9.4 mu M and 24.0 mu M, respe ctively. Linoleic acid, osthenol, falcarindiol and 11(S), 6(R)-dihydro xyoctadeca-9Z,17-diene-12,14-diyn-1-yl acetate exhibited inhibitory ac tivity on COX-1 with IC50 values of 13.3 mu M, 64.3 mu M, 66.0 mu M an d 73.3 mu M.