PERFUSING DENTIN WITH HORSE SERUM OR PHYSIOLOGICAL SALINE - ITS EFFECT ON ADHESION OF DENTIN BONDING AGENTS

Freshly prepared dentine specimens of human teeth were perfused with e ither horse serum or physiologic saline. After application of AllBond2 (R), ART Fond(R), Syntac(R) or an experimental dentine bonding agent c alled P-Bond, a composite cylinder was added and cured at the same tim e. After 1500 thermal cycles with constant imitation of intrapulpal pr essure, the shear bond strengths were measured. Resulting shear bond s trength values were analysed with Students t-test or Mann-Whitney Test . The values for AllBond2(R) were not significantly different. The val ues for ART Bond(R) (P < 0.05) and for Syntac(R) (P < 0.05) were signi ficantly higher if the dentine was perfused with horse serum. For P-Bo nd (P < 0.001) the values were significantly higher if the dentine was perfused with physiologic saline. According to these results it does not seem to be appropriate to take a clear decision as to which of the two perfusing media investigated might be more suitable to imitate in vivo conditions.