FLAVONOL GLYCOSIDES FROM THE SEED COAT OF A NEW MANTECA-TYPE DRY BEAN(PHASEOLUS-VULGARIS L.)

Two flavonol glycosides were isolated from the methanol extracts of '' Prim'', a variety of Phaseolus vulgaris L. (Manteca-type), with a yell ow seed coat color. High-performance liquid chromatography was used to isolate and identify compound 1, kaempferol '-tetrahydroxyflavone)-3- O-beta-D-glucopyranoside, and compound 2, kaempferol D-glucopyranoside -(2-->1)-O-beta-D-xylopyranoside. Concentrations of these compounds we re 49.9 +/- 0.78 mg/100 g and 58.5 +/- 1.67 mg/100 g of fresh whole be an weight, respectively. These two flavonols were the only flavonoids found in Prim and our data indicate that they are responsible for impa rting the yellow color to the seed coat. We were not able to indicate the biochemical effects of the eight genes responsible for seed coat c olor, but we show that G is probably not responsible for producing a f lavonol 3,5-diglycoside as has been hypothesized in the literature. No proanthocyanidins (condensed tannins) were found in Prim dry bean see d coat.