STABILIZATION OF TC-99M D,L-HMPAO PREPARATIONS AS A LEUKOCYTE LABELING AGENT

An attempt was made to use stabilized Tc-99m D,L-HMPAO (S-HMPAO) to la bel leucocytes. The radiochemical purity of Tc-99m D,L-HMPAO, labellin g efficiency of leucocytes, cell viability of labelled leucocytes, and stability of S-HMPAO labelled leucocytes were calculated. In comparis on with commercial Tc-99m D,L-HMPAO (C-HMPAO) without stabilization, i mmediately, at 0.5, 1, 2, 4, and 6 h after HMPAO preparation, the radi ochemical purity of S-HMPAO and the labelling efficiencies of S-HMPAO labelled leucocytes were higher. S-HMPAO is more stable than C-HMPAO a nd can provide higher leucocyte labelling efficiency. S-HMPAO, therefo re, has the potential to replace C-HMPAO as a leucocyte-labelling agen t. (C) 1998 Elsevier Science Ltd. All rights reserved.