CHEMICAL APPROACHES TO INVESTIGATION OF TEMPLATE BIOSYNTHESIS - STUDYOF REPLICATION AND REVERSED TRANSCRIPTION

The chemical approaches to the study of the DNA template biosynthesis by DNA polymerases and reversed transcriptases are considered with the main emphasis on E. coli DNA polymerase I and HIV-1 reversed transcri ptase. Special attention is paid to the comparison of the chemical mod ification, X-ray analysis data, as well as to the quantitative aspects of the DNA polymerases interactions with templates, primers and nucle oside-5'-phosphates. New versions of the catalytically competent label ling developed in the course of investigation of these enzymes are des cribed. The results of application of the primer analogues with photor eactive groups to the study of the replication complexes are presented . Bibliography - 98 references.