SIMULTANEOUS DETERMINATION OF CYANIDE AND THIOCYANATE IN BLOOD BY IONCHROMATOGRAPHY WITH FLUORESCENCE AND ULTRAVIOLET DETECTION

Authors
CHINAKA S TAKAYAMA N MICHIGAMI Y UEDA K
Citation
S. Chinaka et al., SIMULTANEOUS DETERMINATION OF CYANIDE AND THIOCYANATE IN BLOOD BY IONCHROMATOGRAPHY WITH FLUORESCENCE AND ULTRAVIOLET DETECTION, Journal of chromatography B. Biomedical sciences and applications, 713(2), 1998, pp. 353-359
Citations number
19
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
713
Issue
2
Year of publication
1998
Pages
353 - 359
Database
ISI
SICI code
0378-4347(1998)713:2<353:SDOCAT>2.0.ZU;2-N
Abstract
An ion chromatographic method for the simultaneous determination of cy anide and thiocyanate in blood has been developed. After extraction by adding water and methanol to blood, cyanide was derivatized with 2,3- naphthalenedialdehyde and taurine to give a fluorescent product of 1-c yanobenz[f]isoindole. This compound was detected with high sensitivity by fluorometry and the underivatized thiocyanate was detected by ultr aviolet absorption. The detection limits were 3.8 pmol ml(-1) for cyan ide and 86 pmol ml(-1) for thiocyanate, and the recoveries from blood were ca. 83% and ca. 100%, respectively. The proposed method was succe ssfully applied to the analysis of both anions in blood from smokers, non-smokers and fire victims. (C) 1998 Elsevier Science B.V. All right s reserved.