QUANTITATIVE-DETERMINATION OF (-)-2'-DEOXY-3'-THIACYTIDINE (LAMIVUDINE) IN HUMAN PLASMA, SALIVA AND CEREBROSPINAL-FLUID BY HIGH-PERFORMANCELIQUID-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION

A high-performance liquid chromatographic method for the quantitative determination of the HIV reverse transcriptase inhibitor lamivudine (( -)-2'-deoxy-3'-thiacytidine, 3TC, Epivir(R)) in human plasma, saliva a nd cerebrospinal fluid is described. Lamivudine was extracted from sam ples using silica extraction columns prior to reversed-phase high-perf ormance liquid chromatography with ultraviolet detection at 270 nm. Th e method has been validated over the range of 10 (lower limit of quant itation) to 5000 ng/ml using a 0.5-ml sample volume. Between-day and w ithin-day precisions ranged from 3.5 to 9.0%. The assay has been used for the quantitative analysis of lamiwdine in plasma and cerebrospinal fluid of HIV-1 infected patients. (C) 1998 Elsevier Science B.V. All rights reserved.