SYNTHESIS AND EVALUATION OF N-ACETYLNEURAMINIC ACID-BASED AFFINITY MATRICES FOR THE PURIFICATION OF SIALIC ACID-RECOGNIZING PROTEINS

Authors
CICCOTOSTO S KIEFEL MJ ABO S STEWART W QUELCH K VONITZSTEIN M
Citation
S. Ciccotosto et al., SYNTHESIS AND EVALUATION OF N-ACETYLNEURAMINIC ACID-BASED AFFINITY MATRICES FOR THE PURIFICATION OF SIALIC ACID-RECOGNIZING PROTEINS, Glycoconjugate journal, 15(7), 1998, pp. 663-669
Citations number
34
Categorie Soggetti
Biology
Journal title
Glycoconjugate journal → ACNP
ISSN journal
02820080
Volume
15
Issue
7
Year of publication
1998
Pages
663 - 669
Database
ISI
SICI code
0282-0080(1998)15:7<663:SAEONA>2.0.ZU;2-H
Abstract
The synthesis of 2-S-(2-aminoethyl) io-D-glycero-alpha-D-galacto-2-non ulopyranosidonic acid (1) has been successfully achieved from the prec ursors methyl hio-D-glycero-alpha-D-galacto-2-nonulopyranosonate (2) a nd 2-bromo-N-(tert-butoxycarbonyl)-ethylamine (5). Compounds 1 and 2 w ere coupled, via amino and thioglycosidic linkages, respectively, to e poxy-activated Sepharose 6B. The resultant affinity adsorbents have pr oved efficient in purifying the sialic acid-recognizing enzyme Vibrio cholerae sialidase, in a one-step process with yields in the order of 60%.