TAP1 POLYMORPHISM IDENTIFIED IN AFRICAN-AMERICAN GRAVES-DISEASE PATIENTS

Polymorphism in transporter associated with antigen processing (TAP)1 gene has been observed in African American Graves' disease patients. S ingle strand conformational polymorphism has been used to identify var iation for the locus. First-strand cDNA was generated from cell lines obtained by Epstein-Barr virus immortalization. Four variant alleles f or TAP1 have been observed and the products have been sequenced to com pare with the location of observed with SSCP position patterns. Varian ts were detected and compared with substitutions within TAP1 polypepti de which includes changing valine to leucine and three (3) silent subs titutions for glycine, glutamic acid and alanine.