EXOGENOUS DEHYDROEPIANDROSTERONE MODIFIED THE EXPRESSION OF T-HELPER-RELATED CYTOKINES IN NZB NZW F1-MICE/

The onset of lupus-like disease in NZB/NZW F1 mice was correlated with the expression of IL-10 at 4 m of age, and with a sequential enhanced expression of IFN-lr and IL-6 between 6 to 8 m of age. The expression of IFN-gamma and IL-6 was associated with exacerbation of disease sym ptom, production of anti-DNA antibody, and increase in total serum IgG I. Exogenous dehydroepiandrosterone (DHEA) given in animal diet signif icantly prolonged survival, and delayed formation of autoantibody of N ZB/NZW F1 mice as compared to mice fed on control diet. The effect of DHEA paralleled a delay in the expression of IL-10 and IL-6 and an ear lier detection of IL-12 transcripts. Moreover, DHEA-fed mice had highe r serum IgG2a level than control diet-fed mice. Collectively, DHEA may modify the activation of distinct subset of T helper cells in NZB/NZW F1 mice at different phases of disease progression.