Rd. Moreno et al., DEVELOPMENTAL REGULATION OF MOUSE-BRAIN MONOMERIC ACETYLCHOLINESTERASE, International journal of developmental neuroscience, 16(2), 1998, pp. 123-134
Acetylcholinesterase (AChE) molecular forms were studied during mouse
brain development. Mouse embryos expressed a monomeric (G(1)) and a te
trameric (G(4)) AChE form. Our results indicate that G(4) AChE express
ed at embryonic day (ED) 9 and ED15 could be purified by acridinium-Se
pharose chromatography and shared similar biochemical and kinetic prop
erties with the adult form. However, the G(1) form expressed at either
embryonic stage did not bind to acridinium, was not inhibited by exce
ss substrate, and possessed higher K-m and lower V-max values than the
adult G(1) form. Two peripheral anionic binding site inhibitors, fasc
iculin and propidium, had a significantly lower affinity for the monom
eric form at ED9. Results are discussed in terms of the biological sig
nificance of the embryonic G(1) form, and its resemblance to the AChE
activity found, associated with the senile plaques present in the brai
ns of Alzheimer's patients. (C) 1998 ISDN. Published by Elsevier Scien
ce Ltd.